On the participation of singlet oxygen in the acridine orange sensitized photoinactivation of lysozyme.

In a great many cases the participation of singlet oxygen in sensitized photooxigenation processes in solution has been stated Singlet oxygen has sometimes also been assumed to be the important intermediate in photodynamic reactions2. Unfortunatly in most instances a direct proof by spectroscopic methods is practically impossible because of its low emission quantum yield in solution. Therefore evidence has frequently only been produced by more or less specific chemical reactions and by the interpretation of kinetical data, especially of quenching reactions 3.